Protease Culture Condition.

Many of the studies on synthesis of microbial extracellural enzymes have dealt with physiological and environmental factors affecting enzyme production. Synthesis of may extracellular enzymes is governed by concentration of available individual nutrients, catabolic repression and end product inhibition.

In many Organisms protease synthesis is prepared by aminoacids, but the enzymes may also be repressed by glucose as seen in Bacillus subtilis, Aeromonas proteolytica, Serratia sp,

Some extracellular enzymes are inducible peptides and proteins stimulate protease synthesis in Vibrio parahemolyticus, Serratia marcesscens. Imschenetskii et al (1971) demonstrated that with Aspergillus terricola addition of aspartate, methionine, Isoleucine, leucine or valine as the sole source of nitrogen stimulated proteinase production. The culture medium should also have suitable amount of well known nutritive salts of iron, zinc, copper, manganese, calcium, potassium phosphate and magnesium sulphate are particularly valuable additives in promoting the maximum production of enzymes.